Fig. 3.

Multispectral imaging of GE3111 in vivo. Images were recorded 4 h post-IV administration of a 16.67 mg/kg dose. Representative fluorescence images of a mouse sciatic nerve (a) and optic and trigeminal nerves (b) are shown. Nerve location in each image is indicated by an arrow (light white for sciatic nerve, heavy white for trigeminal nerves, yellow for optic nerves), and adjacent tissue structures such as adipose (A) and brain (Br) are annotated. Mice receiving only a single injection of formulation excipients only (no GE3111) were included as control (c). A cross-sectional fluorescence microscopy image of the sciatic nerve depicts binding of GE3111 to the myelin sheath (examples are indicated by yellow arrows) surrounding the nerve axon (d). A cross-sectional image of a sciatic nerve of a control animal is completely dark (e). Normalized spectra of nerve, muscle, and adipose are shown to illustrate spectral separations between tissue types (f).