Fig. 4.

Dose and tissue specific fluorescence in mice following IV administration of GE3111 formulated with 58.5 % distilled water, 30 % 2-HPβCD, 10 % propylene glycol, 1 % PEG-300, and 0.5 % DMSO at the following concentrations (mg/kg): 16.67, 11.67, 6.67, 3.33, 1.05, 0.52, 0.46, and control/formulation excipients only. Sample images of mouse sciatic nerves collected 4 h following a dose of 11.67 mg/kg (a), 3.33 mg/kg (b), or formulation excipient only (c) are shown. The area under the curve for the fluorescence spectrum acquired in sciatic nerve, adjacent muscle, and adipose is calculated and reported as fluorescence (d); n = 3 mice per group. The nerve-to-muscle ratio (mean ± SD) was calculated using the total fluorescence in the sciatic nerve as compared to that of adjacent muscle tissue (e).