Fig. 5.

The kinetics and overall effect of formulation on tissue-specific fluorescence. A single injection of 3.77 mg/kg GE3111 formulated in 58.5 % distilled water, 30 % 2-HPβCD, 10 % propylene glycol, 1 % PEG-300, and 0.5 % DMSO was given 1, 2, 3, 4, 12, and 24 h prior to imaging. (a) The area under the curve for the fluorescence spectrum acquired in sciatic nerve, adjacent muscle, and adipose is calculated and reported as fluorescence (n = 3 mice per group). (b) The nerve-to-muscle ratio was then calculated using the total fluorescence reported in (a) as compared to adjacent muscle tissue. In a separate experiment, mice were given a dose of 3.77 mg/kg GE3111 in formulations varying in 2-HPβCD concentration (e.g., 0 %, 2 %, 10 %, or 30 %), and the images were collected 1 h post-IV administration of GE3111 (c). Tissue specific fluorescence in the sciatic nerve, adjacent muscle, and adipose (n = 3 mice per group) is shown. In (d), nerve-to-muscle ratios of 3.77 mg/kg GE3111 formulated in either 0 % or 30 % 2-HPβCD and given at 1, 2, 3, and 4 h post-injection are shown (n = 3 mice per group).