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. 2012 Sep 11;13(11):1012–1020. doi: 10.1038/embor.2012.133

Figure 2.

Figure 2

Primary preadipocytes and fibroblasts isolated from Hr mutant mice show impaired adipogenesis. (A) Visualization of lipid incorporation by phase-contrast microscopy in differentiated primary preadipocytes isolated from heterozygous (Hrwt/Hhrh) control and homozygous (Hrrh/Hhrh) mice 8 days after differentiation induction. Scale bars, 200 μm (upper panels), 50 μm (lower panels). (B) Visualization of lipid incorporation by phase-contrast microscopy in differentiated primary fibroblasts after 8 days of differentiation. Scale bars, 200 μm (upper panels), 50 μm (lower panels). (C) Relative expression of indicated adipogenic genes in primary preadipocytes using quantitative reverse transcription–polymerase chain reaction (RT–PCR). (D) Relative expression of indicated adipogenic genes in primary fibroblasts using quantitative RT–PCR. Error bars indicate standard deviations. *P<0.05; **P<0.01 (n=4, Student’s t-test). C/EBPβ, CCAAT/enhancer-binding protein-β; HR, hairless; NS, nonsignificant; PPARγ1, peroxisome proliferator-activated receptor-γ1.