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Confluent HCT-8 cells were pretreated for 1 h with DMSO (vehicle), 200 μM imatinib, or 200 μM dasatinib. Cells were then left untreated or treated with 5 μg/ml of Stx2 for 4 h. 150 μg of protein from each whole cell lysate was loaded in each lane. (A) Western blots were performed for phospho-JNKs, phospho-p38, and total ERKs (loading controls). Graphical representation of normalized band intensities are shown for phospho-JNKs (B) and phospho-p38 (C).
