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. 2012 Sep 18;31(21):4204–4220. doi: 10.1038/emboj.2012.262

Figure 2.

Figure 2

HSV1 capsids are wrapped by tubules derived from the plasma membrane. (A) Uninfected HFFF-2 cells were labelled for 2 or 30 min with 10 mg/ml HRP, or 60 min followed by 60 min chase. After fixation, cells were stained with DAB and processed for imaging by EM. (B–J) HFFF-2 cells were infected at a multiplicity of 2 with HSV1 and labelled 11 h after infection with 10 mg/ml HRP for 60 min (B–I), 10 min (J), 2 min (K) or 60 min followed by 60 min chase (L). PM, plasma membrane; ER, endoplasmic reticulum; NM, nuclear membrane; GA, Golgi apparatus; MVB, multivesicular body; Mt, microtubule; CC, clathrin coat; CCP, clathrin-coated pit; Tu, HRP-positive tubule. In (A) (2 min) and (E–K), scale bar=200 nm. In (A) (30 and 60 min+60 min chase) and (B–D, L), scale bar=500 nm.