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. 2012 Sep 18;31(21):4204–4220. doi: 10.1038/emboj.2012.262

Figure 6.

Figure 6

Rab GTPases 5 and 11 are required for efficient HSV1 production. (A, B) HeLa cells transfected with siRNAs as denoted were infected 48 h later with HSV1 at a multiplicity of 5, extracellular virus harvested 16 h later and titre measured. Graphs show titres in relation to those obtained for the negative siRNA (taken as 1), and values are shown as mean±standard error of the mean from three independent experiments. (C) Total cell extracts of siRNA-transfected HeLa cells were analysed by western blotting with antibodies as labelled. (D) HeLa cells transfected with siRNAs for Rab1, Rab5, or Rab11 were assessed for viability. Graph denotes viability in relation to non-transfected cells (taken as 100%). (E) HeLa cells transfected with siRNAs as denoted were infected 48 h later with HSV1 at a multiplicity of 5, and the titre of cell-associated virus was measured 16 h later. Graph shows titres in relation to those obtained for the negative siRNA (taken as 1) and values are shown as mean±standard error of the mean from three independent experiments. (F) Total cell extracts of siRNA transfected, infected HeLa cells, harvested at 16 h, were analysed by western blotting with antibodies as labelled.