Figure 7.

Rab5 and dynamin are required for retrieval of glycoprotein D from the plasma membrane. (A) HeLa cells on coverslips transfected with siRNAs as denoted were infected 48 h later with HSV1 expressing gD-GFP (green) at a multiplicity of 2, fixed 8 or 11 h later and stained with DAPI to detect nuclei (blue). (B) HeLa cells on coverslips transfected with siRNAs as denoted were infected 48 h later with HSV1, and gD antibody uptake experiments were carried out 8 h later. After fixation, cells were stained with anti-mouse secondary antibody to detect anti-gD antibody (red) and DAPI to detect nuclei (blue). (C) HeLa cells infected with HSV1 were incubated for 30 min with dynasore 8 h later (+dyn), or left untreated (−dyn). gD antibody uptake was carried out and cells processed as for (B). Scale bar=10 μm.