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. 2012 Sep 18;31(21):4204–4220. doi: 10.1038/emboj.2012.262

Figure 8.

Figure 8

Rab5 is required for HSV1 envelopment. HeLa cells transfected with siRNAs for Rab5 were infected 48 h later with HSV1 at a multiplicity of 2, and labelled for 30 min with 10 mg/ml HRP 11 h later. Cells were fixed, stained with DAB and processed for EM. (A) An overview of a representative infected, Rab5-depleted cell. (B) Magnified image of juxtanuclear membranes. (C) Magnified image of HRP containing membrane-bound structure. (D) A second example of large HRP-positive structures next to capsids clustering at membranes. (E) Distended membranes with associated capsids. (F) Three serial sections of a cell exhibiting capsids associated with dilated membranes. Sections 2a and 2b are magnified images of section 2, showing the continuum between the individual particles and the cytoplasm (arrowed) that is not obvious in section 3 or the magnified section 3a. In (A), scale bar=10 μm, (B, D), scale bar=2 μm. In (C, E, F) (sections 1–3) scale bar=1 μm. In (F) (sections 2a, 2b & 3a), scale bar=200 nm.