Table 2. Inhibition of dynamin-specific endocytosis reduces the number of HRP-positive wrapping membranes.
HeLa cells infected with HSV1 at a multiplicity of 2 were incubated with or without dynasore (25 μg/ml) for 30 min prior to incubation with HRP (10 mg/ml) for a further 30 min, fixed for EM and stained with DAB. After processing and sectioning, 100 capsids in each sample were scored for the presence of HRP-positive (+ve) or HRP-negative (−ve) membranes, no membrane (free capsids) or primary envelope (ER associated). Numbers represent percentages.