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Chromatin organization of human subtelomeres. (A, B) Conventional ChIP-qPCR was used to assay CTCF, Rad21, SMC3, TRF1, TRF2, histone H3K4me2 and me3, H3K9me2 and me3, and RNAPII binding at various nucleotide positions relative to the TTAGGG repeat tract (position 0) in the XYq subtelomere for either U2OS (A) or HCT116 (B) cell lines. Bar graph represents the average value of percentage of input for each ChIP from three independent ChIP experiments (mean±s.d.).
