Figure 7.

Model of NE-dynein and Eg5 function in prophase centrosome separation. Both Eg5-dependent antiparallel microtubule sliding, as well as minus-end-directed microtubule pulling forces generated by NE-dynein mediate centrosome separation. During late G2/prophase, CENPF and BICD2 are recruited to the NE and in turn recruit/activate dynein at the NE. NE-dynein can bind centrosomal microtubules and pull centrosomes apart through its minus-end-directed motility. Blue and orange arrows indicate the direction of Eg5 and dynein motility, respectively. Red arrows indicate the movement of the centrosomes.