Skip to main content
. 2012 Oct 12;31(21):4153–4164. doi: 10.1038/emboj.2012.274

Figure 1.

Figure 1

RIG-I and STING detect cytosolic infection with Listeria monocytogenes in macrophages. IFNβ in cell-culture supernatants was determined by ELISA 24 h after transfection or 18 h after infection; IFNβ mRNA was determined by qRT-PCR 6 h after transfection or infection. (A) IFNβ, TNF and IL-6 from cell-culture supernatant of bone marrow-derived macrophages (Mø) generated from TRIF−/−, MyD88−/−, TRIF/MyD88−/− and wild-type (wt) littermate mice. In all, 2 × 105 Mø after infection with 2 × 106 CFU wt Listeria, LLO-deficient Listeria mutant (Δhly) (MOI of 10) or 2 × 108 of HKLM or mock treatment. (B) IFNβ in cell-culture supernatant of RIG-I−/−, STING−/− or wt Mø infected with Listeria. LPS (500 ng/ml) as positive control. (C) Time kinetics of IFNβ mRNA from wt, STING−/− or RIG-I−/− Mø after infection. NS, not significant; **P=0.01, ***P=0.001 (unpaired Student’s t-test). Data are representative of at least three separate experiments (mean and s.d. of triplicates).