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. 2012 Oct 12;31(21):4153–4164. doi: 10.1038/emboj.2012.274

Figure 3.

Figure 3

Secreted Listeria DNA is recognized by RIG-I via the RNA-polymerase III pathway. (A–C) IFNβ in cell-culture supernatants of (A) wt, TRIF−/− or MyD88−/− Mø transfected with seDNA (20 ng/105 cells), (B) wt, STING−/−, RIG-I−/− or MDA5−/− Mø transfected with seDNA (20 ng/105 cells) or (C) wt, MDA5−/− or RIG-I−/− Mø transfected with seDNA (20 ng/105 cells) or lysDNA (2 μg/105 cells). (D) IFNβ mRNA assessed by qRT-PCR in wt macrophages after transfection with seRNA (10 ng/105 cells) or seDNA (20 ng/105 cells), lysRNA (1 μg/105 cells) or lysDNA (2 μg/105 cells) after treatment with the RNA polymerase III inhibitor (ML-60218, 4 μM) for 10 h prior to transfection and during the incubation time. NS, not significant; *P=0.05, **P=0.01, ***P=0.001 (unpaired Student’s t-test). Data are representative of at least three separate experiments (mean and s.d. of triplicates).