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Roscovitine significantly enhances vesicle mobility. A, Comparison of the time course of FRAP of SypI-EGFP-labeled vesicles in WT neurons preincubated for 30 min with 100 μm roscovitine (solid diamonds) and control neurons (open triangles, mean ± SEM). Roscovitine significantly increased the fraction of mobile vesicles. B, as in A but in TKO neurons treated (open diamonds) or untreated (solid triangles) with roscovitine. No effect of roscovitine on the FRAP time course is evident.
