Table 1.
The IC50 values (nM) of influenza neuraminidase inhibition.a
| Virus | OC | ZA | 3a | 3c | 4a | 4c |
|---|---|---|---|---|---|---|
| A/WSN/33 (H1N1) | 2.6±1.4 | 2.9±1.4 | 2.4±0.8 | 3.0±2.0 | 0.09±0.05 | 1.1±0.1 |
| A/WSN/33/H275Y (H1N1)b | 477±181 | 1.6±0.4 | 993±666 | 1679±43 | 0.4±0.1 | 25.1±6.6 |
| NIBRG14 (H5N1) | 0.73±0.15 | 4.8±0.2 | 0.52±0.26 | 2.2±0.6 | 0.04±0.01 | 17.4±8.6 |
| A/Taiwan/3446/2002 (H3N2) | 3.1±0.9 | 13.8±2.7 | 1.8±0.06 | 5.9±0.1 | 0.3±0.06 | 131.7±16.6 |
| A/Udorn/1972 (H3N2) | 2.9±0.4 | 22.9±7.8 | 1.6±0.6 | 3.8±1.1 | 0.35±0.05 | 114±20.3 |
| B/Taiwan/70641/2004 | 36±6.9 | 32.1±8.4 | 73.6±14 | 80.9±12.7 | 2.1±0.7 | 124.8±32.4 |
a
A fluorescent substrate, 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (MUNANA) was used to determine the IC50 values that are compound concentrations causing 50% inhibition of different influenza neuraminidase enzymes. Data are shown as mean ± SD of three experiments.
b
A/WSN H275Y virus by oseltamivir selection.