Figure 2.

Effects of IFNγ on hNSCs and mNSCs. In (A) photomicrographs of undifferentiated hNSCs (Wicell h9 line) and mNSCs are given. A βIII-tubulin and GFAP co-staining under control conditions and after IFNγ treatment is shown. The upper to panels show mNSCs, the lower two panels show hNSCs. In mNSCs, IFNγ typically leads to a strong up-regulation of neuronal (βIII-tubulin) and glial (GFAP) markers with many cells simultaneously expressing both markers (arrows and the merged picture). In hNSCs no IFNγ-induced up-regulation of these markers was detected. In (B) gene expression levels of βIII-tubulin, GFAP, and SHH in undifferentiated hNSCs (Wicell h9 line) either under control conditions or after IFNγ treatment are shown. The results demonstrate that there is, also on mRNA level, no IFNγ-induced up-regulation of cell type-specific markers.