Figure 3.

Down-stream gene regulation and population size of hNSCs after IFNγ exposure and in vivo levels of IFNγ in patients CSF samples. In (A) gene expression levels in hNSCs (Wicell h9 line) of i-NOS (inducible nitric oxid synthase), IRF-9 (interferon regulatory factor 9), c-Myc, MHC1 (major histocompatibility complex 1), and Stat 1 (signal transducers and activators of transcription 1) are given either under control conditions or after IFNγ treatment. Results show that IFNγ significantly up-regulates important down-stream factors except of SHH. Values are means ± standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times. In (B) population size of hNSCs (Wicell h9 line) over 7 days is measured by means of an MTT-assay. Population sizes of two groups, hNSCs under control conditions or after IFNγ treatment, are compared. Results show that IFNγ does not affect the population size of hNSCs. Values are means ± standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times. In (C) amounts of IFNγ in clinical CSF samples are measured by ELISA in comparison to CSF samples of control patients. For statistical testing of the multiple sclerosis group, 3 control patients were compared with 3 multiple sclerosis patients [unpaired two-tailed t-test, value is mean ± standard error of mean (SEM)].