Table 2.

miRNAs included in the RT-qPCR analysis regarding their metastatic association.^†

Selected miRNAs by microarray analysis
		Microarray‡		RT-qPCR‡
No.	miRNA	Fold changes	P	Fold changes	P
1	miR-10b	-2.29	0.001	-3.91	<0.0001
2	miR-19a	-2.76	0.001	-1.94	<0.0001
3	miR-19b	-1.68	0.003	-1.71	<0.0001
4	miR-21	+1.65	0.035	+1.23	0.986
5	miR-29a	-1.78	0.003	-1.98	0.0004
6	miR-29b	-3.36	0.001	-4.28	<0.0001
7	miR-29c	-3.63	0.001	-4.00	<0.0001
8	miR-100	-2.40	0.025	-1.87	0.007
9	miR-101	-2.17	0.001	-3.57	0.001
10	miR-126	-2.76	0.001	-2.60	0.002
11	miR-130a	-1.76	0.013	-1.78	0.019
12	miR-143	-2.51	0.015	-2.44	0.011
13	miR-145	-2.80	0.005	-2.56	0.001
14	miR-148a	-2.10	0.009	-1.65	0.039
15	miR-192	-2.98	0.013	-5.13	0.001
16	miR-194	-3.40	0.010	-7.05	0.0002
17	miR-195	-2.31	0.021	-2.55	0.054
18	miR-223	+2.83	0.013	-1.02	0.824
19	miR-370	+11.3	0.001	+1.43	0.905
20	miR-451	+4.79	0.013	+1.60	0.403
21	miR-494	+5.73	0.001	+1.17	0.063
22	miR-575	+7.87	0.001	see Note§	-
23	miR-630	+15.2	0.001	see Note§	-
24	miR-638	+5.93	0.001	+1.56	0.030
Selected miRNAs from a ccRCC-specific miRNA panel 17
1	miR-141	-	-	-3.91	<0.0001
2	miR-155	-	-	+1.52	0.267
3	miR-200c	-	-	-2.62	0.004
4	miR-210	-	-	-1.54	0.063
5	miR-224	-	-	+1.38	0.484
6	miR-514	-	-	-3.78	0.007
Selected miRNAs after literature search
1	let-7f	Gottardo et al. 14		see Note§
2	miR-20a	Chow et al. 29		-1.40	0.002
3	miR-26a	Liu et al. 31, Chow et al. 30, Heinzelmann et al. 20		-2.11	<0.0001
4	miR-106b	Chow et al. 30, Slaby et al. 19		see Note§
5	miR-125b	Liu et al. 31		see Note§
6	miR-127	Saito et al .28		-1.43	0.091
7	miR-215	Nakada et al. 15		-7.28	0.007
8	miR-296	Juan et al. 18		+3.55	0.001

^†miRNAs investigated in this study were chosen on the basis of the microarray-based selection of metastamirs in the present study, a specific panel of differentially expressed miRNAs in renal cell carcinoma described in a previous study ¹⁷ and literature data as indicated. ^‡Fold changes in metastatic to primary renal cell carcinoma samples (see Figure 1 and Supplementary Material: Table S1). Symbols: +, up-regulation; -, down-regulation. ^§miRNAs in italics showed Cq values >35 in RT-qPCR of the pooled samples and were therefore excluded in subsequent analyses.