Fig. 4.

MSC proliferation and migration are marginally affected by poly(I:C) treatment. MSC were treated with saline (MSC) or 4 μg/ml poly(I:C) (MSC-IC) for 24 h and washed to remove poly(I:C). Poly(I:C)-treated MSC are designated as MSC-IC. Cells were then analyzed by MTT assay, migration assay, and Western blotting. A: cells were plated in 24-well plates (10⁵ cells/well). MTT assays were performed 1, 2, and 3 days after plating. OD, optical density. B: transwell migration assay for MSC and MSC-IC. Migration index is defined as the ratio of migrated MSC-IC to migrated MSC. C: cells were harvested 2 days after plating and processed for SDS-PAGE and Western blot analysis. Proteins (45 μg) were loaded in each lane. The ratios of chemokine receptor CXCR4, integrin β1, integrin α5, and integrin αV to GAPDH are graphed. n = 3; **P < 0.01 and ***P < 0.001 vs. untreated MSC.