Fig. 2.

Prolonged treatments with MGO led to channel inhibition. A: currents were induced by pinacidil (Pin) from a cell treated with the sham solution overnight. Glib, glibenclamide. B: pinacidil-induced currents became much smaller in another cell treated with 1 mM MGO for 3 h. C: overnight MGO treatment further reduced the pinacidil-induced currents. D: summary of the current density changes in control and MGO treatment (21 h, 1 mM). **P < 0.01. E: currents were recorded from cells treated with 1 mM MGO for different time periods and described as a function of time with a single exponential equation. Current density was calculated by dividing the current amplitude by the whole cell capacitance in each cell. Calculated time constant is 3.46 h. Calibrations are 5 nA/2 min for A, 3 nA/1 min for B, and 3 nA/1.2 min for C.