Fig. 4.
Action of MGO on the 3′-untranslated regions (UTRs) of KATP channel mRNAs. A10 cells transfected with Kir6.1–3′-UTR or SUR2B-3′-UTR containing luciferase reporter vector (pmirGLO) were treated with different concentrations of MGO for 5 h, followed by the measurement of the luciferase activity. The ratio of firefly luciferase activity (experimental reporter): renilla luciferase activity (control reporter) was calculated for each well. The well ratio of each sample was normalized to the control well ratio and is represented on the y-axis as the relative luciferase activity in response to different MGO treatments along with the control. A: summary of the effect of different concentrations (10, 100, and 300 μM) of MGO on the luciferase activity of Kir6.1–3′-UTR containing luciferase reporter vector. B: summary of the effect of different concentrations of MGO (30, 100, and 300 μM) on the luciferase activity of SUR2B-3′-UTR containing luciferase reporter vector. *P < 0.05; ***P < 0.001 (n = 5 experiments).