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. 2012 Sep 19;303(10):C1079–C1089. doi: 10.1152/ajpcell.00162.2012

Fig. 4.

Fig. 4.

Selective PDE1 inhibition with 8MM-IBMX increases the STH frequency and hyperpolarizes guinea pig DSM cell membrane potential. A: original current-clamp recording illustrating that 8MM-IBMX (10 μM) increased the STH frequency, and hyperpolarized the DSM cell membrane potential. Subsequent addition of paxilline (1 μM) abolished the STHs and depolarized the membrane potential. A portion of the recording is shown on an expanded time scale. B: summary data showing that 8MM-IBMX (10 μM) significantly increased STH frequency and had no effect on the STH amplitude (n = 5, N = 5; *P < 0.05; NS, nonsignificant). C: summary data showing that 8MM-IBMX (10 μM) significantly hyperpolarized the cell membrane potential and that subsequent inhibition of BK channels with paxilline (1 μM) abolished the hyperpolarizing effect of PDE1 inhibition and depolarized the membrane potential (n = 8, N = 8; *P < 0.05).