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. 2012 Nov 8;2:785. doi: 10.1038/srep00785

Figure 3. EMT phenotype induced by CS knockdown greatly increases tumor cell migration and proliferation in vitro.

Figure 3

(A) Wound healing migration assay of CS knockdown cells. Cells as indicated were cultured until confluent then performed scratched wound healing migration assay. (B) Boyden chamber migration assay of CS knockdown cells. Cells as indicated were seeded in Boyden chamber and incubated for 6 h. The migrated cells were stained and scored. (C) Matrigel invasion assay of CS knockdown cells. Cells as indicated were plated in invasion chamber and incubated for 8 h. The invaded cells were stained and counted. (D) MTT cell growth assay of CS knockdown cells. Cells as indicated were cultured in 96-well plates and then performed the MTT assay according to standard procedures. (E) BrdU incorporation assay of CS knockdown cells. Cells as indicated were cultured in 96-well plates and then carried out the Cell Proliferation ELISA, BrdU (colorimetric) assay according to the manufacturer′s protocols. (F) Colony formation assay of CS knockdown cells. Cells as indicated were cultured in 6-well plates for 6 days. The grown colonies were stained and scored. (G) Soft agar colony formation assay of CS knockdown cells. Cells as indicated were suspended in 0.35% top agar and overlaid on 0.7% bottom agar, then cultured in 6-well plates for 12 days. The grown colonies were counted. The plotted data were averaged from three independent experiments and the bars represent mean ± SD.