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. 2012 Nov 8;2:785. doi: 10.1038/srep00785

Figure 4. EMT phenotype induced by CS knockdown dramatically accelerates tumor malignancy in vivo.

Figure 4

(A) In vivo tumor growth assay of CS knockdown cells. Indicated cells were subcutaneously inoculated into the backs of NOD/SCID mice for 20 or 60 days. The mice were sacrificed and the tumors were removed and examined. (B) In vivo tumor metastasis assay of CS knockdown cells. Indicated cells were intravenously injected into the tail vein of NOD/SCID mice for 20 days. The mice were sacrificed and examined for tumor metastases. Red arrowheads indicate the hearts. (C) Histological and immunohistochemical analyses of lung and heart in the in vivo tumor metastasis assay. The analyses were carried out using H&E staining and immunostaining with antibody specific for vimentin.