Table 1.

Nucleotide sequences of mutagenic primers.

Primer	Primer Sequence (5′-3′)	Nucleotide substitution	Amino acid substitution
D1-G106R	TTCCTTTCCGAAGAGACGACAGCCATTGCCCCAGCC	GGG-CGT	Gly-Arg
D1-L107D	TTCCTTTTCCGAAATCCCCACAGCCATTGCCCCAG	CTC-GAT	Leu-Asp
D1-G106RL107D	CTTCCTTTTCCGAAATCCCGACAGCCATTGCCCCAGCC	GGA-CGG CTC-GAT	Gly-ArgLeu-Asp
D1-K310D	AGCCACTTCGTCCTCTAGCTTGAATGAGCCTGTGC	AAA-GAC	Lys-Asp
D1-E311K	TCAGCCACCTTTTTCTCTAGCTTGAATGAGCCTGTGC	GAA-AAG	Glu-Lys
D1-K310DE311K	GGGTCTCAGCCACCTTGTCCTCTAGCTTGAATGAGCCTGTGC	AAA-GAC GAA-AAG	Lys-AspGlu-Lys
D1-P364Q	GCCTCAATGTTGACCTGTTTTTCTTTGTCAGTGACTATGGG	CCA-CAG	Pro-Gln

Mutated nucleotides are shown in bold.