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. Author manuscript; available in PMC: 2012 Nov 8.
Published in final edited form as: Oncogene. 2011 Jun 13;31(1):60–67. doi: 10.1038/onc.2011.215

Figure 3.

Figure 3

Dissociated WAP-Int3/WC/R26 PI-MECs contribute to mammary gland regeneration when transplanted with normal MECs. (a) A total of 2 × 106 dissociated WAP-Int3/WC/R26 MECs failed to regenerate a gland upon transplantation into a cleared mammary fat pad of a nu/nu female host. (b) When 2 × 105 dissociated WAP-Int3/WC/R26 MECs were mixed with 1 × 105 or 2 × 105 normal MECs, PI-MECs (blue cells) contributed to the resulting complete outgrowths. (c) Higher magnification image of a whole-mounted mammary gland taken from a 8-day pregnant mouse 22 days following inoculation with a mix of 2 × 105 WAP-Int3/WC/R26 and 2 × 105 normal MECs demonstrating contribution of WAP-Int3/WC/R26 PI-MECs to both end buds (black arrow) and developing side branches (white arrow). (d) Cross-section of an end bud in a developing WAP-Int3/WC/R26 and normal MECs chimeric gland demonstrating contribution of the WAP-Int3/WC/R26 PI-MECs to the body of the end bud, but not the cap cells (arrows). Inset is a higher magnification of the cap cells of the same image. (e) Cross section of a duct from a chimeric gland demonstrating presence of WAP-Int3/WC/R26 PI-MECs’ progeny. (f) Cross-section of and 8-day pregnant chimeric gland demonstrating contribution of WAP-Int3/WC/R26 PI-MECs to the developing acinar structures. Scale Bars: a and b = 1000 µm; c = 200 µm; d and e = 150 µm; f = 50 µm.