Skip to main content
. Author manuscript; available in PMC: 2012 Nov 8.
Published in final edited form as: Oncogene. 2011 Jun 13;31(1):60–67. doi: 10.1038/onc.2011.215

Table 2.

Results of cellular transplantations

Inoculation Mammary
outgrowths		 Tumors β Gal+
outgrowths/
tumors
1 × 105 MECs 4/6 0/6 0/4
2 × 105 Int3 : 1 × 105 MECs 4/7 0/7 4/4
2 × 105 Int3 : 2 × 105 MECs 7/13 0/13 6/7
2 × 106 Int3 cells 0/20 0/20 NA
1 × 105 Int3 tumor cells 0/4 4/4 0/4

Abbreviation: MECs, mammary epithelial cells.

2 × 105 WAP-Int3/WC/R26 (Int3) MECs were mixed with 1 × 105 or 2 × 105 normal MECs from Balb/c mice and inoculated into cleared mammary fat pads of nu/nu female mice. PI-MECs from WAP-Int3/WC/R26 mice (marked by β Gal) contributed to the resulting outgrowths. WAP-Int3/WC/R26 never regenerated a gland on their own when 2 × 106 cells were inoculated. WAP-Int3/WC/R26 tumor cells only gave rise to β Gal− tumors. There was no significant difference between take rates in either Wap-Int3/WC/R26:wild-type MECs-mixed inoculations and that observed for wild-type MECs alone, but both mixed inoculations and MECs alone had significantly higher take rates than Wap-Int3 cells alone (P = 0.002, 0.0015 and 0.001, respectively).