Table 1.

Polymerase chain reaction primer sequences

Gene	Sequence	Annealing temperature (°C)	Product size (bp)
PPAR-γ2	Forward: 5′-GAG CAT GGT GCC TTC GCT GA Reverse: 5′-AGC AAG GCA CTT CTG AAA CCG A	52.5	564
Osteopontin	Forward: 5′-GCT CTA GAG CAC AAT CTT CTA GCC CC Reverse: 5′-GAC GTC GAC TGA CCT CAG TCC GTA AGC C	53	319
HNF-3β	Forward: 5′-AGC AGC AAC ATC ATC ACA GC Reverse: 5′-AAA GTT CCC CCA ATG TTT CC	52.5	313
CK-18	Forward: 5′-TCA AGA ACT GGG GCA CTA CC Reverse: 5′-CAT GTC TTT GCT GGC TTC AA	52	383
CK-19	Forward: 5′-TCC TCC TCA CCA TGA CTT CC Reverse: 5′-CGA ATC TTC ACC TCC AGC TC	55	358
Albumin	Forward: 5′-CTT CAA AGC CTG GGC AGT AG Reverse: 5′-AGT AAT CGG GGT GCC TTC TT	55	395
AFP	Forward: 5′-TGG AGA AGT GCT CCC AGT CT Reverse: 5′-GCA GTG GTT GAT ACC GGA GT	57.5	359
HGF	Forward: 5′-ACA CAT CTG TGG GGG ATC AT Reverse: 5′-TGG TGC TGA CTG CAT TTC TC	55.5	396
c-Met	Forward: 5′-TGT GCA TTC CCC ATC AAA TA Reverse: 5′-CAC AGG ATA GGA ACC CAG GA	51	373
MMP-2	Forward: 5′-GGA CAG TGA CAC CAC GTG AC Reverse: 5′-TCC AGT TAA AGG CAG CGT CT	55	240
GAPDH	Forward: 5′-AGA CAG CCG CAT CTT CTT GT Reverse: 5′-TAC TCA GCA CCA GCA TCA CC	56	323

AFP, α-fetoprotein; CK-18, cytokeratin-18; CK-19, cytokeratin-19; HGF, hepatocyte growth factor; HNF-3β, hepatocyte nuclear factor-3β; MMP-2, matrix metalloprotease-2; PPAR-γ2, peroxisome proliferator-activated receptor-γ2.