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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Stem Cells. 2012 Apr;30(4):591–598. doi: 10.1002/stem.1061

Figure 1.

Figure 1

A subpopulation of LRCC is not quiescent and undergoes active cell division. (A): Asymmetric cell division (ACD) with nonrandom chromosomal cosegregation. Each chromosome in some stem cells contains one template DNA strand that is conserved throughout multiple ACDs (orange mark) and preferentially segregates into the daughter stem cell. (B): Isolation of live LRCC (Materials and Methods and Supporting Information Fig. S1A, S1B). (C–G): Ki67 expression, phospho-histone-3 expression, cell cycle phases, doubling times, and key cell cycle check point genes expression in LRCC versus non-LRCC are not statistically different indicating that LRCCs are not quiescent. Abbreviations: dUTP, 2′-deoxyuridine 5′-triphosphate; FACS, fluorescence-activated cell sorting; LRC, label-retaining cell; LRCC, label-retaining cancer cell; CCNA2, cyclin A2; CCND1, cyclin D1; CCND2, cyclin D2; CCND3, cyclin D3; CCNE1, cyclin E1; CDC2, cell division control protein 2; CDK2; cyclin-dependent kinase 2; CDK4, cyclin-dependent kinase 4; CDK6, cyclin-dependent kinase 6.