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. Author manuscript; available in PMC: 2012 Nov 8.
Published in final edited form as: J Immunol. 2011 Jun 1;187(1):291–297. doi: 10.4049/jimmunol.1100528

FIGURE 2.

FIGURE 2

Reduced expression of CCL19, CCL21, and IL-7 in the spleen of SIRPα MT mice. Expression of CCL19 (Ccl19), CCL21 (Ccl21), or CXCL13 (Cxcl13) mRNA (A) and IL-7 (Il7) mRNA (B) in the spleen of WT or SIRPα MT mice was evaluated by quantitative PCR. The level of expression of each mRNA was normalized to that of GAPDH mRNA and presented as fold increase relative to the value for WT mice. Data are means ± SE for a total of 10 mice per group in three independent experiments. C, Frozen sections of the spleen from WT or SIRPα MT mice were double-stained with pAbs to CCL19 (left panels, red), CCL21 (middle panels, red), or CXCL13 (right panels, red) and an mAb to B220 (left and middle panels, green) or Thy1.2 (right panels, green). Scale bar, 200 μm. D, Frozen sections of the spleen from WT or SIRPα MT mice were stained with mAbs to gp38 (red) and B220 (green). Scale bar, 200 μm (left panels). The area for gp38-positive region was measured per each image. Data are means ± SE for a total of four (WT) or three (MT) mice per group (right panel). *p < 0.05, **p < 0.01 (Student t test).