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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Microbiol Methods. 2012 Aug 30;91(2):222–230. doi: 10.1016/j.mimet.2012.08.012

Figure 1.

Figure 1

Schematic of procedure for suspension cell infection with C. trachomatis serovar F/Cal-I-13, purification of chlamydial forms and vesicle isolation. Multiple concentrations of OptiPrep™ density gradient medium used in this study are as indicated. Abbreviations: EB, elementary body; RB, reticulate body; PF, persistent form; SPG, sucrose-phosphate-glutamate buffer; PBS, phosphate buffered saline.