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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Microbiol Methods. 2012 Aug 30;91(2):222–230. doi: 10.1016/j.mimet.2012.08.012

Figure 4.

Figure 4

Characteristics of the persistent form fraction. (a) Western blot analysis of isolated chlamydial forms with monoclonal antibodies specific to chlamydial MOMP, OmcA, CPAF and RpoB. Sample loading normalized based on total protein. All samples prepared in Laemmli sample buffer (1:1, v:v) supplemented with 5% 2-Mercaptoethanol, 10 mM dithiothreitol and heated for 10 min at 100 °C. (b) ATP transport assays conducted with isolated persistent forms. ATP indicates control transport assays containing 20-times the determined Km for C. trachomatis nucleoside phosphate transporter 1. All data normalized by adjusting maximum transport to 100%. Experiments were performed in triplicate on three independent chlamydial preparations. Abbreviations: EB, elementary body; RB, reticulate body; PF, persistent form; ATP, adenosine-5'-triphosphate.