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Analysis of vesicle populations by Western blot. (a) Relative enrichment of chlamydial LPS-containing vesicles results in a decrease of host derived vesicles. (b) Purity of the vesicle fraction and the association of putative virulence factors with the chlamydial vesicle population. Vesicle samples prepared in Laemmli sample buffer (1:1, v:v) supplemented with 5% 2-Mercaptoethanol, 10 mM dithiothreitol and heated for 10 min at 100 °C. Abbreviation: EB, elementary body.
