Figure 2.

Molecular-sieve Sephadex G-10 chromatography (fourth) of the cell sap of buckwheat leaves. The crude cell sap was prepared from the leaves of buckwheat intermittently exposed to 50 μm Al in 0.5 mm CaCl₂, pH 4.5, for 10 d. Dilute HClO₄ solution, pH 4.6, was used as the eluant, and fractions (3 mL each) were collected at a flow rate of 0.72 mL min⁻¹. □, Al; ⋄, oxalic acid.