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13C-NMR spectra of Al-oxalate (1:25) complex (A) and the purified cell sap of buckwheat leaves (B). Buckwheat was intermittently exposed to 50 μm Al in 0.5 mm CaCl2, pH 4.5, for 10 d. The crude cell sap was purified four times by Sephadex G-10 column chromatography. Spectra were measured at 150.8 MHz. See Methods for the purification process and measurement conditions.
