Figure 1.

Representative standard curve for the target sequence peptide, native arginine peptide and elongated peptide. Signal inhibition is only seen towards the selected peptide sequence, and no reactivity (signal inhibition) towards either the elongated peptide or native arginine peptide implying specificity for the citrulline-rich peptide. The signal was assessed as the optical density at 450 nm, subtracting the background at 650 nm, as a function of peptide concentration. Measurement is the mean of a double determination measurement.