Figure 1.

Analytical work-flow diagram for studying the basic protein changes during skeletal muscle aging. Shown is a flow diagram of the pre-fractionation approach using offgel electrophoresis in order to enrich for muscle proteins with a basic pI-value. Shown are representative silver-stained 2D gels of the acidic vs. alkaline range of proteins following separation by offgel electrophoresis. The subproteomic analysis of young adult vs. aged muscle proteins was then performed by 2D gels employing a pH 6.0–11.0 range.