Table I.
List of basic proteins with a changed abundance in aged rat gastrocnemius muscle.
| Spot no. | Accession no. | Name of identified protein | Peptides matched | Peptide sequence | Coverage sequence (%) | Molecular mass (kDa) | Isoelectric point (pI) | Mascot score | Fold-change |
|---|---|---|---|---|---|---|---|---|---|
| 1 | gi|38259206| | Mitochondrial creatine kinase | 4 | REVENVAITALEGLKG KLSEMTEQDQQRL RGIWHNYDKT KVPPPLPQFGRK |
10 | 47.89 | 8.64 | 69 | 1.5 |
| 2 | gi|55741544| | Ubiquinol cytochrome-c reductase core protein 2 | 7 | KLPNGLVIASLENYAPLSRI RRWEVAALRS KAVAFQNPQTRI RIIENLHDVAYKN KEVAEQFLNIRG RGGLGLAGAKA KAVAQGNLSSADVQAAKN |
18 | 48.42 | 9.16 | 81 | 1.5 |
| 3 | gi|6671762| | Muscle creatine kinase | 8 | KLNYKPQEEYPDLSKH KVLTPDLYNKL KDLFDPIIQDRH KTDLNHENLKG KGGDDLDPNYVLSSRV KFEEILTRL RGTGGVDTAAVGAVFDISNADRL RLGSSEVEQVQLVVDGVKL |
26 | 43.25 | 6.58 | 168 | −1.5 |
| 4 | gi|62664602| | Glyceraldehyde 3-phosphate-dehydrogenase (GAPDH) | 5 | KLVINGKPITIFQERD RGAAQNIIPASTGAVKAVGKV KVIPELNGKL KLISWYDNEYGYSNRV RVVDLMAYMASKE |
19 | 36.18 | 7.6 | 84 | −1.6 |
| 5 | gi|56188| | GAPDH | 2 | KVIHDNFGIVEGLMTTVHAITATQKT KLISWYDNEYGYSNRV |
11 | 36.10 | 8.43 | 126 | −1.6 |
| 6 | gi|56188| | GAPDH | 10 | KVGVNGFGRI KLVINGKPITIFQERD KIVSNASCTTNCLAPLAKV RGAAQNIIPASTGAAKA KVIPELNGKLTGMAFRV KLTGMAFRV RVPTPNVSVVDLTCRL KLISWYDNEYGYSNRV RVVDLMAYMASKE RVVDLMAYMASKE |
32 | 36.09 | 8.43 | 185 | −1.9 |