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CD1d-based aAPCs can be used to expand human NKT cells. CD161+ T cells were isolated from PBMC using magnetic bead separation. The cells were stimulated biweekly with irradiated PBMC pulsed with 100 ng/mL α-GalCer (10:1 ratio) or a-GalCer-loaded aAPC+αCD28 (1:1 ratio). Data shown are after 2 rounds of stimulation. PBMC, peripheral blood mononuclear cells.
