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. 2012 Nov 5;4:e5035add8caff4. [Version 1] doi: 10.1371/5035add8caff4

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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IP assay of dysferlin and MG53. — MG53interacts with dysferlin in mouse skeletal muscle. Extracts from wild-type mouse skeletal muscle were subjected to IP with polyclonal anti-MG53 antibodies or polyclonal anti-dysferlin antibodies. Immunoprecipitated proteins were subjected to SDS-PAGE and visualized on immunoblots treated with the same antibodies that were used for IP.