Identification of MG53-binding region of dysferlin.

The dysferlin C2A domain associates with MG53. Constructs encoding dysferlin deletion mutants were used for co-IP assays, and the results of these experiments are shown on the right. Deletion mutants encoding c-myc-tagged dysferlin mutants and FLAG-tagged full-length MG53 were co-expressed in COS-7 cells. IP and immunoblotting were performed using antibodies against the c-myc and FLAG tags. MG53 was co-immunoprecipitated with full-length dysferlin and the dysferlin mutants that lacked the C-terminus, but not with the dysferlin mutants that lacked the N-terminus.