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. 2012 Nov 5;4:e5035add8caff4. [Version 1] doi: 10.1371/5035add8caff4

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Membrane repair assay of myofiber transfected with dysferlin-GFP and RFP-MG53. — RFP-C242A MG53 perturbed the accumulation of dysferlin at wound sites in the sarcolemma. A. Dysferlin-GFP was simultaneously expressed with RFP-tagged wild-type MG53 or the RFP-C242A-MG53 mutant in mouse skeletal muscle. Arrowheads indicate sites of membrane injury, which were induced with a two-photon laser microscope. Dysferlin-GFP accumulated at the injury site in the presence of RFP-wild-type MG53, but no obvious accumulation of dysferlin-GFP was observed in the presence of the RFP-C242A-MG53 mutant. Scale bar, 10 mm. B.Time course fluorescence intensity (n=3) at wounded sites versus time. For every image taken, the fluorescence intensity of dysferlin-GFP at the site of the damage (circle of 5 mm in diameter) was measured with Zeiss LSM5 Image Examinar software. Data are means ± standard deviation.