Figure 1.

Pam₃CSK₄ induces MCP-1 expression by a TLR2-dependent, but MyD88-independent pathway. (A) Raw264.7 cells were treated with Pam₃CSK₄ (100 ng/ml) for the indicated duration of times. MCP-1 mRNA expression was determined by RT-PCR and normalized to β-actin control. (B) BMDMs were isolated from wild-type or TLR2 knockout (KO) mouse bone marrow and treated with Pam₃CSK₄ (100 ng/ml) for the indicated duration of times. MCP-1 mRNA expression was determined by RT-PCR and normalized to β-actin control. (C) Raw264.7 cells were transfected with either control or TLR2 or MyD88 siRNA (200 pM) for 24 h. Cells were stimulated with vehicle or Pam₃CSK₄ (100 ng/ml) for 6 h. TLR2, MyD88 and MCP-1 mRNA expression was determined by RT-PCR and normalized to β-actin control. (D) Raw264.7 cells were transfected with control or TRIF siRNA (200 pM) for 24 h. Cells were stimulated with vehicle of Pam₃CSK₄ (100 ng/ml) for 6 h. TRIF and MCP-1 mRNA expression was determined by RT-PCR and normalized to β-actin control.