Figure 5.

GSK3β is a downstream molecule for Akt in TLR2-mediated MCP-1 expression. (A) Raw264.7 cells were treated with Pam₃CSK₄ (100 ng/ml) in the presence or absence of LiCl (20 mM) for the indicated duration of times. GSK3β phosphorylation was determined by western blotting using α-phospho-GSK3β antibody (S9) and normalized to GSK3β total protein. (B) Raw264.7 cells were treated with Pam₃CSK₄ (100 ng/ml) for 6 h in the presence or absence of LiCl (20 mM). MCP-1 mRNA expression was determined by RT-PCR and normalized to β-actin control. (C) Raw264.7 cells were treated with Pam₃CSK₄ (100 ng/ml) in the presence or absence of LY294002 (10 μM) for the indicated duration of times. GSK3β phosphorylation was determined by western blotting using α-phospho-GSK3β antibody (S9) and normalized to GSK3β total protein.