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ALDH+ Tca8113 cells form tumorspheres in vitro. Isolated single ALDH+ and ALDH− Tca8113 cells were cultured in serum-free medium. (A and D) Cells were monitored the next day after being seeded in the plates. Tumorspheres were observed after a 5- or 10-day incubation of the ALDH+ cells (B and C), while tumorspheres were not noted in the cultured ALDH− cells (E and F).
