Figure 4.

Determination of roscovitine-induced apoptosis in transient silencing of SSAT in Caco-2 cells. (A) Following transfection of Caco-2 cells with SSAT siRNA 1.6, 2.7 and their combination for 48 h, roscovitine treatment was performed for 24 h. SSAT silencing was confirmed by SSAT immunoblotting. Total protein (30 mg) was loaded into each well. β-actin was used as loading control. (B) CDK inhibitor-induced apoptosis was evaluated in SSAT transiently silenced cells. Columns represent the means ± SD of two independent experiments with at least four replicates (^*p<0.05).