TABLE 1.
Influence of Iowa and Milano mutations on apoA-I physical properties
| Parameter | Wild-type | Iowa | Milanoa |
|---|---|---|---|
| Change in α-helix content (%)b | - | −12 | −2 |
| Relative ANS binding | 1.0 | 1.5 | 1.6 |
| Free energy of denaturation (kcal/mol)c | 3.5 ± 0.1 | 2.4 ± 0.1 | 2.4 ± 0.2 |
a
The data for the monomer (reduced) form are from ref. (14).
b
The α-helix content of wild-type apoA-I was 49 ± 5% and varied somewhat with sample history.
c
CD was employed to monitor the loss of α-helix content when apoA-I was exposed to increasing concentrations of GdmCl.