Induction of CYP2E1 and CYP2A5 by chronic ethanol feeding in WT, Cyp2e1
–/–, and Cyp2a5
–/– mice. After 3 weeks of ethanol feeding, induction of CYP2E1 and CYP2A5 was measured as described in Materials and Methods section. (A) Activities of CYP2E1 and CYP2A5 in WT and Cyp2e1
–/– mice. (B) Western blotting analyses for CYP2E1 and CYP2A5 in WT and Cyp2e1
–/– mice. *p < 0.05, compared with Cont group; #p < 0.05, compared with WT EtOH group. Cont, Control; EtOH, Ethanol. (C) Activities of CYP2E1 and CYP2A5 were inhibited by CMZ. *p < 0.05, compared with Cont group; #p < 0.05, compared to EtOH group. (D) Activities of CYP2E1 and CYP2A5 in WT and Cyp2a5
–/– mice. *p < 0.05, compared with Cont group; #p < 0.05, compared with WT EtOH group. (E) Western blotting analyses for CYP2E1 and CYP2A5 in WT and Cyp2a5
–/– mice. *p < 0.05, compared with Cont group. (F) Ethanol oxidation in microsomes isolated from WT and Cyp2a5
–/– mice fed dextrose or ethanol liquid diet. *p < 0.05, compared with Cont group; #p < 0.05, compared with WT EtOH group.