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Assessment of endothelial integrity by immunohistochemistry. Aortic sections were stained with three endothelial markers, anti-VEGF, anti- FLT-1 and anti-FLK-1 and the epitopes were revealed by DAB staining I) Negative control, no primary antibodies, II) Treated sections: A) control, B) US, C) OPT and D) US + OPT; The short arrows mark the endothelium. Magnification is 40x and representative sections from two experiments are shown.
